Recombinant Mouse SOD2/MnSOD protein (ab113179)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Molecular weight
      25 kDa including tags
    • Amino acids
      1 to 222
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Superoxide dismutase >2500units/mg
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab113179 is purified by multi-step chromatography.
  • Form
    Liquid
  • Additional notes
    Superoxide dismutase >2500units/mg
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 1.5% Glycerol, 0.47% Sodium chloride

General Info

  • Alternative names
    • Indophenoloxidase B
    • IPO B
    • IPOB
    • Manganese containing superoxide dismutase
    • Manganese SOD
    • Manganese superoxide dismutase
    • Mangano superoxide dismutase
    • Mn SOD
    • Mn superoxide dismutase
    • MNSOD
    • MVCD6
    • SOD 2
    • SOD2
    • SODM_HUMAN
    • Superoxide dismutase [Mn] mitochondrial
    • Superoxide dismutase [Mn], mitochondrial
    • Superoxide dismutase 2 mitochondrial
    see all
  • Function
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

Images

  • SDS-PAGE analysis:
    Lane 1: MW marker
    Lane 2: ab113179 at 2μg
    Lane 3: ab113179 at 5μg.
  • All lanes : Anti-Superoxide Dismutase 2 antibody

    Lane 1 : Molecular Weight Markers
    Lane 2 : Mouse Brain Tissue Extract
    Lane 3 : Recombinant Mouse SOD2/MnSOD protein (ab113179)

References

ab113179 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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In regards to your first question, we used an anti-SOD antibody for the western blot image. However the particular product we used is not offered by us for sale. We do o...

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While I feel that such testing would be valuable, unfortunately at this time we do not have a peptide testing discount program. We do have an abreview program in which you might post the results of such tests on ...

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