Recombinant mouse Thrombopoietin protein (ab73295)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesMouse
    • SequenceSPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
    • Molecular weight19 kDa
    • Additional sequence informationThrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton.

Specifications

Our Abpromise guarantee covers the use of ab73295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50 as determined by the dose-dependant stimulation of MO7e cells is < 1 ng/ml, corresponding to a specific activity of 1 x 106 IU/mg.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab73295 was purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    No additives.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • C mpl ligand
    • C-mpl ligand
    • Megakaryocyte colony stimulating factor
    • Megakaryocyte colony-stimulating factor
    • Megakaryocyte growth and development factor
    • Megakaryocyte stimulating factor
    • MGC163194
    • MGDF
    • MKCSF
    • ML
    • MPL ligand
    • MPLLG
    • Myeloproliferative leukemia virus oncogene ligand
    • Prepro thrombopoietin
    • THCYT1
    • THPO
    • Thrombopoietin
    • Thrombopoietin nirs variant 1
    • TPO
    • TPO_HUMAN
    see all
  • FunctionLineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • Involvement in diseaseDefects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
  • Sequence similaritiesBelongs to the EPO/TPO family.
  • DomainTwo-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant mouse Thrombopoietin protein (ab73295)

ab73295 has not yet been referenced specifically in any publications.

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