Additional sequence informationThrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe ED50 as determined by the dose-dependant stimulation of MO7e cells is < 1 ng/ml, corresponding to a specific activity of 1 x 106 IU/mg.
% SDS-PAGE. ab73295 was purified by proprietary chromatographic techniques.
Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
C mpl ligand
Megakaryocyte colony stimulating factor
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
Megakaryocyte stimulating factor
Myeloproliferative leukemia virus oncogene ligand
Thrombopoietin nirs variant 1
FunctionLineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Involvement in diseaseDefects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
Sequence similaritiesBelongs to the EPO/TPO family.
DomainTwo-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.