The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological activity: Mimics insulin effects in differentiating 3T3-L1 cells.
% SDS-PAGE. Purity >95% as determined by SDS-PAGE and HPLC
ENDOTOXIN: <0.1 EU/µg protein (LAL test)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in PBS to 1mg/ml
Pre B cell colony enhancing factor
Pre B cell colony enhancing factor 1
Pre B cell enhancing factor
Pre-B cell-enhancing factor
Pre-B-cell colony-enhancing factor 1
FunctionCatalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Tissue specificityExpressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
PathwayCofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
Sequence similaritiesBelongs to the NAPRTase family.