Overview

Description

  • NatureRecombinant
  • SourceCell Culture
  • Amino Acid Sequence
    • AccessionP27467
    • SpeciesMouse
    • SequenceSLAVGPQYSS LSTQPILCAS IPGLVPKQLR FCRNYVEIMP SVAEGVKAGI QECQHQFRGR RWNCTTVSNS LAIFGPVLDK ATRESAFVHA IASAGVAFAV TRSCAEGSAA ICGCSSRLQG SPGEGWKWGG CSEDIEFGGM VSREFADARE NRPDARSAMN RHNNEAGRQA IASHMHLKCK CHGLSGSCEV KTCWWSQPDF RTIGDFLKDK YDSASEMVVE KHRESRGWVE TLRPRYTYFK VPTERDLVYY EASPNFCEPN PETGSFGTRD RTCNVSSHGI DGCDLLCCGR GHNARTERRR EKCHCVFHWC CYVSCQECTR VYDVHTCK
    • Amino acids19 to 352
    • Additional sequence informationab81484 is a monomeric glycoprotein containing 334 amino acid residues. This protein is the mature full length (aa19-352), it does not contain the signal peptide from aa 1-18.

Specifications

Our Abpromise guarantee covers the use of ab81484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 for this effect is 0.4-0.6 ng/ml.

  • Applications

    Cell differentiation

    SDS-PAGE

    Cellular Activation

  • Purity70 - 90% by HPLC.
    Greater than 75% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised
  • Additional notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute to a concentration of 0.1 mg/ml. Once reconstituted product is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C.

General Info

  • Alternative names
    • Protein Wnt 3a Precursor
    • Protein Wnt-3a
    • Wingless type MMTV integration site family member 3A
    • Wnt 3a
    • wnt3a
    • WNT3A protein
    • WNT3A_HUMAN
    see all
  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificityModerately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt

Recombinant mouse Wnt3a protein images

  • Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

References for Recombinant mouse Wnt3a protein (ab81484)

This product has been referenced in:
  • Grol MW  et al. P2X7 nucleotide receptor signaling potentiates the Wnt/ß-catenin pathway in cells of the osteoblast lineage. Purinergic Signal 12:509-20 (2016). Read more (PubMed: 27206526) »
  • Xie W  et al. Sox2 modulates Lef-1 expression during airway submucosal gland development. Am J Physiol Lung Cell Mol Physiol 306:L645-60 (2014). Mouse . Read more (PubMed: 24487391) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

I have talked to the lab and they have told me that the purification techniques are proprietary, so all I know it that if was purified by HPLC.

Please let me know if you have any further questions.

Abreviews
Application Other
we use wnt3a as a positive control for the TOPflash luciferase assay and it works well!
Username

Abcam user community

Verified customer

Submitted Apr 08 2015

Abreviews
Application Functional Studies
This Wnt3a was used to treat mouse embryonic stem cells, which are quite responsive to Wnt3a.
We treated our cells for 24 hours with 200 ng/mL Wnt3a and then examined Wnt/beta-catenin target gene (e.g. Axin2) expression by qRT-PCR or Tcf/Lef activity by using a dual-luciferase reporter system (Super 8X-TOP/FOP-Flash).

The Wnt3a-mediated activation of the Wnt/beta-catenin pathway, as assessed by target gene activation or Tcf/Lef reporter activity, was not appreciably different from that observed when recombinant Wnt3a from other vendors was used.
Username

Abcam user community

Verified customer

Submitted Dec 05 2014



Unfortunately this note you see on the online product datasheet is the most current information we have regarding availability of ab81484. There are perhaps some production troubles slowing down release of a new batch. I suggest checking peri...

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Thank you for contacting us.

I have obtained the following information for you:

For ab136932: This protein is of invertebrate origin, specifically the cnidarian, Hydra. Thus, it is neither of mouse nor human origin. We will add this i...

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Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you. I now have further information to share.

As discussed over the phone, unfortunately we are currently experiencing some problems in...

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Sorry for misunderstanding. I was suggesting to order the antibody; we will then be placingyour orderin "wait for stock" status so in a month time when this protein will be available again you will be getting an dispatch confirmation email of...

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Thank you for contacting us.

Our lab has been very busy preparing this protein however the yield is very low at the moment. I am sorry we are unable to confirm the exact availability date of this product. t can take weeks.

I would ...

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Thank you for contacting us.

I can confirm that I have sent an email to my colleague in lab and now waiting for their reply.

Many thanks for having patience!

This protein should be reconstituted in water to a concentration of 1.0 mg/ml. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"