Recombinant Mycobacterium tuberculosis (Hsp-65) protein (ab73800)

Overview

  • Product nameRecombinant Mycobacterium tuberculosis (Hsp-65) protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SequenceHHHHHHGSAKTIAYDEEARRGLERGLNALADAVKVTLGPKGRNVVLEKKW GAPTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATV LAQALVREGLRNVAAGANPLGLKRGIEKAVEKVTETLLKGAKEVETKEQI AATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRF DKGYISGYFVTDPERQEAVLEDPYILLVSSKVSTVKDLLPLLEKVIGAGK PLLIIAEDVEGEALSTLVVNKIRGTFKSVAVKAPGFGDRRKAMLQDMAIL TGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVEGAGDTDAIAGR VAQIRQEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHR IEDAVRNAKAAVEEGIVAGGGVTLLQAAPTLDELKLEGDEATGANIVKVA LEAPLKQIAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLAAGVADP VKVTRSALQNAASIAGLFLTTEAVVADKPEKEKASVPGGGDMGGMDF
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab73800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 2.5mM Potassium chloride, 130mM Sodium chloride, 10mM Sodium phosphate, pH 7.4

  • ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • 60 kDa chaperonin 2
    • 65 kDa antigen
    • Antigen A
    • Cell wall protein A
    • groEL
    • GroEL protein 2
    • GroEL2
    • Heat shock protein 65
    • Hsp65
    see all
  • RelevanceMycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Cellular localizationCytoplasmic

References for Recombinant Mycobacterium tuberculosis (Hsp-65) protein (ab73800)

ab73800 has not yet been referenced specifically in any publications.

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