Recombinant Mycobacterium tuberculosis TB Ag85A protein (ab124604)

Overview

  • Product name
    Recombinant Mycobacterium tuberculosis TB Ag85A protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected BTI-TN-5B1-4 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Mycobacterium tuberculosis
    • Sequence
      ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAHHHHHH
    • Molecular weight
      33 kDa including tags
    • Amino acids
      43 to 338
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab124604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    > 95 % SDS-PAGE.
    ab124604 is purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names
    • 85 A
    • 85A
    • Ag85A
    • Antigen 85 A
    • Antigen 85 complex A
    • Antigen 85A
    • Fbp A
    • FbpA
    • Fibronectin binding protein A
    • Mpt 44
    • Mpt44
    • Mycobacterium tuberculosis antigen 85A
    • Mycolyl transferase 85 A
    • Mycolyl transferase 85A
    • Rv3804c
    • Secreted antigen Ag85A
    see all
  • Relevance
    Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly.
  • Cellular localization
    Secreted; cell wall. Cytoplasm

Recombinant Mycobacterium tuberculosis TB Ag85A protein images

  • 15% SDS-PAGE showing ab124604 (3 µg).

References for Recombinant Mycobacterium tuberculosis TB Ag85A protein (ab124604)

This product has been referenced in:
  • Mancha-Agresti P  et al. Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-?, IL-6, and TNF-a Cytokines after Intranasal Immunization. Front Microbiol 8:1263 (2017). Functional Studies ; Mouse . Read more (PubMed: 28744263) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.
Please find below the answers to your questions:

1.
I am happy to confirm that this protein (ab124604)is Antigen 85 sub unit A.

2.
a) ab73632 is the full length protein (aa 1 to 325)
b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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