Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab54064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Inhibition Assay

    SDS-PAGE

    ELISA

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 20mM Glutathione, 100mM Sodium chloride, pH 8

General Info

  • Alternative names
    • 5''-cyclic phosphodiesterase 10A
    • cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A
    • cAMP and cAMP-inhibited cGMP 3''
    • FLJ11894
    • HSPDE 10A
    • HSPDE10A
    • PDE 10A
    • PDE10_HUMAN
    • Pde10a
    • Phosphodiesterase 10A
    • Phosphodiesterase 10A1
    see all
  • FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
  • Tissue specificityAbundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • DomainThe tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
    Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
  • Cellular localizationCytoplasm. Located mostly to soluble cellular fractions.
  • Information by UniProt

References for Recombinant PDE10A protein (ab54064)

ab54064 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab54064.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"