Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells

Specifications

Our Abpromise guarantee covers the use of ab80327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 8339 pmol/min/µg. Assay conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 1 mM MnCl2, 200 uM cAMP, 2.5 kU 5' nucleotidase, 37oC, 20min.
  • Applications

    SDS-PAGE

    Functional Studies

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 5''-cyclic phosphodiesterase 10A
    • cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A
    • cAMP and cAMP-inhibited cGMP 3''
    • FLJ11894
    • HSPDE 10A
    • HSPDE10A
    • PDE 10A
    • PDE10_HUMAN
    • Pde10a
    • Phosphodiesterase 10A
    • Phosphodiesterase 10A1
    see all
  • Function
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
  • Tissue specificity
    Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Domain
    The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
    Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
  • Cellular localization
    Cytoplasm. Located mostly to soluble cellular fractions.
  • Information by UniProt

Recombinant PDE10A protein images

  • ab80327 on 10% SDS-PAGE, Coomassie staining, 10µg.

References for Recombinant PDE10A protein (ab80327)

ab80327 has not yet been referenced specifically in any publications.

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