Recombinant PDGF BB protein (ab9706)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Additional notesBiological Activity is determined by the dose-dependent stimulation of the proliferation of BALB/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute with water to a concentration of 0.1 – 1 mg/ml. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. If reconstituting to a concentration less than 0.1 mg/ml add a carrier protein such as 0.1% BSA.

General Info

  • Alternative names
    • Becaplermin
    • PDGF 2
    • PDGF B chain
    • PDGF subunit B
    • PDGF-2
    • PDGF2
    • Pdgfb
    • PDGFB_HUMAN
    • Platelet derived growth factor 2
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto-oncogene c-Sis
    • SIS
    • SSV
    see all
  • Information by UniProt

References for Recombinant PDGF BB protein (ab9706)

ab9706 has not yet been referenced specifically in any publications.

Product Wall


The lab let me know that this antibody (ab84265, detection antibody) can be used with ab9704 as capture antibody and ab9706 as protein standard.

Thank you for contacting us.

For ab73229, I was not able to receive any additional information for using the protein infunctional studies. As for ab9706, the procedure is as follows:

Procedure: (Previously reconstituted in water an...

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Yes, either HSA or BSA will be fine to use in the reconstitution of ab9706. We would also recommend a quick spin in a microcentrifuge prior to opening the vial to make sure the protein gets down to the bottom of the vial, then using sterile H2O and add...

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Thank you for contacting us. Both PDGF BB protein ab9706 (human) and ab51925 (mouse) have been assessed for activity by dose-dependent stimulation of the proliferation of Balb/c 3T3 cells with ED50 of 1.0-3.0 ng/ml and 2 ng/ml resepectively. They are b...

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Thank you for your enquiry. Human PDGF-BB, ab9706, is reconstituted in water: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and store...

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Thank you for your enquiry. Yes, we would highly recommend centrifuging the vial before opening, as some of the contents may have attached to the cap region during transport. To reconstitute this product, you can dilute in water. The volume of wate...

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Thank you for your enquiry. Yes, this product can be diluted water. The volume of water depends on the concentration of the product itself. Then, you can dilute the product in any other buffer in order to place it in working aliquots. Have you purchase...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"