The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe biological activity of ab87461 was determined in a chemotaxis assay using a Boyden chamber with freshly isolated swine PBMC monocytes using a Percoll gradient enrichment method. Biological activity typically peaks at 100 ng/mL
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile phosphate buffered saline containing at least 0.1% carrier protein.
Beta chemokine RANTES
Beta chemokine RANTES precursor
C C motif chemokine 5
Chemokine (C C motif) ligand 5
Chemokine CC Motif Ligand 5
Eosinophil chemotactic cytokine
Regulated upon activation normally T expressed and presumably secreted
Small inducible cytokine A5
Small inducible cytokine A5 (RANTES)
Small inducible cytokine subfamily A (Cys Cys) member 5
Small-inducible cytokine A5
T cell specific protein p288
T cell specific RANTES protein
T cell-specific protein P228
T-cell-specific protein RANTES
FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificityT-cell and macrophage specific.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
Post-translational modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells. The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.