Overview

Description

  • Nature
    Recombinant
  • Source
    Saccharomyces cerevisiae
  • Amino Acid Sequence
    • Species
      Pig
    • Sequence
      SPYASDTTPC CFSYLSRPLP RAHLQEYFYT SSKCSMAAVV FITRKNRQVC ANPEKKWVRE YINTLEMS
    • Amino acids
      1 to 68

Specifications

Our Abpromise guarantee covers the use of ab87461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The biological activity of ab87461 was determined in a chemotaxis assay using a Boyden chamber with freshly isolated swine PBMC monocytes using a Percoll gradient enrichment method. Biological activity typically peaks at 100 ng/mL
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1.0X PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile phosphate buffered saline containing at least 0.1% carrier protein.

General Info

  • Alternative names
    • Beta chemokine RANTES
    • Beta chemokine RANTES precursor
    • C C motif chemokine 5
    • CCL 5
    • CCL5
    • CCL5_HUMAN
    • Chemokine (C C motif) ligand 5
    • Chemokine CC Motif Ligand 5
    • D17S136E
    • EoCP
    • Eosinophil chemotactic cytokine
    • MGC17164
    • RANTES(4-68)
    • Regulated upon activation normally T expressed and presumably secreted
    • SCYA 5
    • SCYA5
    • SIS delta
    • SIS-delta
    • SISd
    • Small inducible cytokine A5
    • Small inducible cytokine A5 (RANTES)
    • Small inducible cytokine subfamily A (Cys Cys) member 5
    • Small-inducible cytokine A5
    • T cell specific protein p288
    • T cell specific RANTES protein
    • T cell-specific protein P228
    • T-cell-specific protein RANTES
    • TCP 228
    • TCP228
    see all
  • Function
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificity
    T-cell and macrophage specific.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab87461 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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