Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Molecular weight62 kDa
    • Amino acids232 to 546
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab71666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity is 59 nmol/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Double stranded RNA activated protein kinase;
    • E2AK2_HUMAN
    • eIF-2A protein kinase 2
    • EIF2AK1
    • EIF2AK2
    • Eukaryotic translation initiation factor 2 alpha kinase 2
    • Eukaryotic translation initiation factor 2-alpha kinase 2
    • HGNC:9437
    • Interferon induced double stranded RNA activated protein kinase
    • Interferon inducible elF2 alpha kinase
    • Interferon inducible RNA dependent protein kinase
    • Interferon-induced, double-stranded RNA-activated protein kinase
    • Interferon-inducible RNA-dependent protein kinase
    • MGC126524
    • P1/eIF-2A protein kinase
    • P1/eIF2A protein kinase
    • p68 kinase
    • PKR
    • PPP1R83
    • PRKR
    • Protein kinase interferon inducible double stranded RNA dependent
    • Protein kinase RNA activated
    • Protein kinase RNA-activated
    • Protein phosphatase 1 regulatory subunit 83
    • Serine/threonine protein kinase TIK
    • Tyrosine protein kinase EIF2AK2
    see all
  • FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
  • Information by UniProt

Recombinant PKR protein images

References for Recombinant PKR protein (ab71666)

This product has been referenced in:
  • Chen YC  et al. Polo-like kinase 1 is involved in hepatitis C virus replication by hyperphosphorylating NS5A. J Virol 84:7983-93 (2010). Functional Studies . Read more (PubMed: 20534861) »

See 1 Publication for this product

Product Wall



This protein does still contain the N-terminal tag.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"