The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityProtein A+G binds to all IgG subclasses (from various mammalian species).
This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G.
The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody.
Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.
Protein A+G binds to all IgG subclasses (from various mammalian species).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Endotoxin level is <0.1 EU/mg of Protein A/G.
ReconstitutionReconstitute in water to a concentration of 5 mg/ml.
Cellular localizationProtein A: Secreted; cell wall.
Protein G: Cell Wall and Secreted
References for Recombinant Protein A + Protein G (ab52213)