Overview

  • Product nameRecombinant Protein A + Protein G
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • TagsHis tag N-Terminus
    • Additional sequence informationThe molecular weight including the His tag is 59.7 kDa

Specifications

Our Abpromise guarantee covers the use of ab52213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityProtein A+G binds to all IgG subclasses (from various mammalian species).
  • Applications

    SDS-PAGE

    Affinity purification

  • Purity> 98 % SDS-PAGE.

  • FormLyophilised
  • Additional notes


    This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.

    Protein A+G binds to all IgG subclasses (from various mammalian species).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is <0.1 EU/mg of Protein A/G.

  • ReconstitutionReconstitute in water to a concentration of 5 mg/ml.

General Info

  • Cellular localizationProtein A: Secreted; cell wall. Protein G: Cell Wall and Secreted

References for Recombinant Protein A + Protein G (ab52213)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"