Recombinant Protein G (ab155724)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Protein G
See all Protein G proteins and peptides -
Purity
> 98 % SDS-PAGE.
Lyophilized from 650 µL bulk protein in a 0.2 µm filtered solution of 0.1 M NaH2PO4, pH8.5 with 10% trehalose as protectant. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Predicted molecular weight
31 kDa -
Tags
His tag N-Terminus -
Additional sequence information
Contains only one single cysteine at the C-terminus, which is designed for single point attachment coupling for immobilization.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab155724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.5
Constituents: 1.1998% Sodium Phosphate Monobasic, 10% D-(+)-Trehalose dihydrate -
ReconstitutionReconstitute with sterile deionized water to a final concentration of 20mg/ml.
General Info
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Alternative names
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
see all -
Relevance
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Cell Wall and Secreted
Images
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SDS-PAGE analysis of reduced ab155724 stained overnight with Coomassie Blue.
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Immobilized Recombinant Protein G, His Tag (ab155724) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (ab246197) with a linear range of 0.01-0.313 μg/mL
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155724 has been referenced in 1 publication.
- Yasunaga AB et al. Quantitative interpretation of cell rolling velocity distribution. Biophys J 120:2511-2520 (2021). PubMed: 33932434