Recombinant rat Biliverdin Reductase protein (ab91622)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesRat

Specifications

Our Abpromise guarantee covers the use of ab91622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiliverdin Reductase Assay: Positive (> 180 units/mg) 1 unit of biliverdin reductase will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purified by Multi-Step Chromatography. > 95% pure as determined by SDS-PAGE and Western Blot analysis.
  • FormLiquid
  • Additional notesBiliverdin Reductase Assay: Positive (> 180 units/mg) 1 unit of biliverdin reductase will transform 1 nanomole of biliverdin to bilirubin in an NADPH dependent reaction at pH 8.5 at 37°C in 1 minute.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, 5mM DTT, 1mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • BIEA_HUMAN
    • Biliverdin IX alpha reductase
    • Biliverdin reductase A
    • Biliverdin-IX alpha-reductase
    • BLVR
    • BLVR A
    • Blvra
    • BVR
    • BVR A
    • BVRA
    • Zinc metalloprotein
    see all
  • FunctionReduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
  • Tissue specificityLiver.
  • PathwayPorphyrin-containing compound metabolism; protoheme degradation.
  • Involvement in diseaseHyperbiliverdinemia
  • Sequence similaritiesBelongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant rat Biliverdin Reductase protein images

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight marker
    Lane 2: 0.5 µg ab91622
    Lane 3: 1.0 µg ab91622
    Lane 4: 2.0 µg ab91622
    Lane 5: 5.0 µg ab91622
  • All lanes : Biliverdin Reductase Polyclonal Antibody

    Lane 1 : Molecular weight markers
    Lane 2 : ab91622 at 0.1 µg
  • Biliverdin Reductase Activity: 1 µg of ab91622 was incubated with biliverdin in the presence of NADPH at pH 8.5, 37°C. Product formation was monitored with a spectrometer at 450 nm.

References for Recombinant rat Biliverdin Reductase protein (ab91622)

ab91622 has not yet been referenced specifically in any publications.

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