The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityFully biologically active by its ability to phosphorylate STAT3 in several cells lines.
% SDS-PAGE. ab123755 is purified by proprietary chromatographic techniques. Purity by SDS-PAGE and gel filtration is greater than 99%.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituent: 0.025% Sodium carbonate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in sterile ddH2O or 0.4 % NaHCO3 adjusted to pH 8-9 to a concentration of 100 µg/ml. This solution can then be diluted into other aqueous buffer, preferably in the presence of carrier protein.
Reconstituted CNTF should be stored at 4°C for 2-7 days and as working aliquots at -20°C for future use. Avoid freeze/thaw cycles.
Ciliary neurotrophic factor
FunctionCNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.