Recombinant Rat Cystatin C protein (ab63285)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesRat
    • SequenceMRGSHHHHHH GMAS-GTSRPP PRLLGAPQEA DASEEGVQRA LDFAVSEYNK GSNDAYHSRA IQVVRARKQL VAGINYYLDV EMGRTTCTKS QTNLTNCPFH DQPHLMRKAL CSFQIYSVPW KGTHTLTKSS CKNA

Specifications

Our Abpromise guarantee covers the use of ab63285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Please filter the product by an appropriate sterile filter before using it in cell culture.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.03 M Acetate buffer, pH 4

  • ReconstitutionAdd 200ul of deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/mL. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • AD 8
    • AD8
    • Amyloid angiopathy and cerebral hemorrhage
    • ARMD11
    • bA218C14.4
    • bA218C14.4 (cystatin C)
    • Cst 3
    • Cst3
    • CST3 protein
    • Cystatin 3
    • Cystatin-3
    • Cystatin-C
    • Cystatin3
    • CystatinC
    • CYTC_HUMAN
    • Gamma trace
    • Gamma-trace
    • HCCAA
    • MGC117328
    • Neuroendocrine basic polypeptide
    • Post gamma globulin
    • Post-gamma-globulin
    see all
  • FunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • Involvement in diseaseAmyloidosis 6
    Macular degeneration, age-related, 11
  • Sequence similaritiesBelongs to the cystatin family.
  • Post-translational
    modifications
    The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant Rat Cystatin C protein images

  • LANE 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane.

References for Recombinant Rat Cystatin C protein (ab63285)

ab63285 has not yet been referenced specifically in any publications.

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