Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Rat
    • Molecular weight
      38 kDa
    • Amino acids
      2 to 335

Specifications

Our Abpromise guarantee covers the use of ab123225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Enzyme activity: 90 unit/µl (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37°C in 60 min.)
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.60
    Constituents: 2.28% Potassium chloride, 0.02% DTT, 0.79% Tris HCl, 0.003% EDTA, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DNA directed DNA polymerase beta
    • DNA pol beta
    • DNA polymerase beta
    • DNA polymerase beta subunit
    • DPOLB_HUMAN
    • MGC125976
    • Pol B
    • Pol beta
    • POLB
    • Polymerase (DNA directed) beta
    see all
  • Function
    Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • Sequence similarities
    Belongs to the DNA polymerase type-X family.
  • Domain
    Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • Post-translational
    modifications
    Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • Cellular localization
    Nucleus, Cytoplasm
  • Information by UniProt

Images

  • SDS-PAGE of ab123225

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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