Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Rat
    • Sequence
      MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
    • Molecular weight
      16 kDa
    • Amino acids
      16 to 155

Specifications

Our Abpromise guarantee covers the use of ab126927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is = 0.1 ng/ml, in the presence of 10 µg/ml heparin, corresponding to a specific activity of = 1 x 107 units/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.
    Greater than 95% by SDS-PAGE gel and HPLC analyses.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C.

    pH: 7.20
    Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 0.2% CHAPS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.2 to a concentration of =0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

General Info

  • Alternative names
    • Acidic fibroblast growth factor
    • aFGF
    • Beta endothelial cell growth factor
    • Beta-endothelial cell growth factor
    • ECGF
    • ECGF beta
    • ECGF-beta
    • ECGFA
    • ECGFB
    • Endothelial Cell Growth Factor alpha
    • Endothelial Cell Growth Factor beta
    • FGF 1
    • FGF alpha
    • Fgf1
    • FGF1_HUMAN
    • FGFA
    • Fibroblast growth factor 1
    • Fibroblast Growth Factor 1 Acidic
    • GLIO703
    • HBGF 1
    • HBGF-1
    • HBGF1
    • Heparin binding growth factor 1
    • Heparin binding growth factor 1 precursor
    • Heparin-binding growth factor 1
    see all
  • Function
    The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Cellular localization
    Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt

References

ab126927 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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