Recombinant Rat G protein alpha (mutated Q212 L + D280 N) (ab90410)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesRat

Associated products

Specifications

Our Abpromise guarantee covers the use of ab90410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

  • FormLiquid
  • Additional notes


    In contrast to all other known G protein alpha D/N mutants, the exchange of Asp280 to Asn280 in GGsalphaS does not lead to an inactivation in nucleotide binding. Mutation of Gln212 to Leu212 inhibits the intrinsic GTPase activity, resulting in a constitutively activated GsalphaS. This mutation also increases the GDP-affinity of GsalphaS.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

General Info

  • Alternative names
    • Adenylate cyclase stimulating G alpha protein
    • AHO
    • C20orf45
    • Extra large alphas protein
    • GNAS complex locus
    • GNAS1
    • GPSA
    • GSA
    • GSP
    • Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
    • Guanine nucleotide binding protein G(s) alpha subunit
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas
    • guanine nucleotide regulatory protein
    • NESP
    • Neuroendocrine secretory protein
    • PHP1A
    • PHP1B
    • PHP1C
    • POH
    • RP4-543J19.4
    • Secretogranin VI
    • XLalphas
    see all
  • RelevanceGTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.
  • Cellular localizationCell Membrane

References for Recombinant Rat G protein alpha (mutated Q212 L + D280 N) (ab90410)

ab90410 has not yet been referenced specifically in any publications.

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