Recombinant rat IL-17A + IL-17F protein (ab109681)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant rat IL-17A + IL-17F protein -
Biological activity
The ED50 of ab109681, as determined by its ability to induce IL-6 production in NIH-3T3 cells, is 7.28ng/mL. -
Purity
> 98 % SDS-PAGE.
Purity Greater than 98% determined by reducing and non-reducing SDS-PAGE. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Rat -
Sequence
MAVLIPQSSVCPNAEANNFLQNVKVNLKVLNSLSSKASSRRPSDYLNRST SPWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILV LKREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS -
Predicted molecular weight
15 kDa -
Amino acids
18 to 150
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Amino Acid Sequence 2
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Species
Rat -
Sequence
MAVLIPQSSVCPNAEANNFLQNVKVNLKVLNSLSSKASSRRPSDYLNRST SPWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILV LKREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS -
Predicted molecular weight
15 kDa -
Amino acids
29 to 161
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Specifications
Our Abpromise guarantee covers the use of ab109681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. The formulation is from 0.02% TFA before product is lyophilized
General Info
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Alternative names
- Ctla 8
- CTLA8
- Cytokine ML1
see all -
Relevance
IL-17F stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Cellular localization
Secreted
Images
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SDS PAGE analysis of ab109681 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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ab109681 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Rat IL-17AF is a heterodimer with a predicted total MW of 30.7 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab109681 has not yet been referenced specifically in any publications.