The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab73839 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Additional notesLigand binding to LRPAP1 is Ca2+ dependent and e.g. lipid receptors can be released from LRPAP1 by a buffer containing 10mM EDTA (cf. Bajari et al. 2005, see ref. below). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab73839 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
39 kDa receptor-associated protein
Alpha 2 macroglobulin receptor associated protein
Alpha 2 MRAP
Alpha-2-macroglobulin receptor-associated protein
Lipoprotein receptor associated protein
Low density lipoprotein receptor related protein associated protein 1
Low density lipoprotein receptor-related protein-associated protein 1
Low density lipoprotein related protein associated protein 1
Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein
low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1)
FunctionInteracts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
Involvement in diseaseNote=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
Sequence similaritiesBelongs to the alpha-2-MRAP family.
Cellular localizationEndoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.