Recombinant rat M-CSF protein (Active) (ab56288)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ8JZQ0
    • SpeciesRat
    • SequenceMEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP
    • Molecular weight36 kDa
    • Amino acids33 to 186
    • Additional sequence informationRecombinant Rat M-CSF is a 36.2 kDa homodimeric protein consisting of two 155 amino acid polypeptide subunits.

Specifications

Our Abpromise guarantee covers the use of ab56288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 5.0 ng/ml, corresponding to a specific activity of ≥ 2 x 105 units/mg.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers.

General Info

  • Alternative names
    • Colony stimulating factor 1
    • Colony stimulating factor 1 (macrophage)
    • Colony stimulating factor macrophage specific
    • CSF 1
    • CSF-1
    • CSF1
    • CSF1_HUMAN
    • Csfm
    • Lanimostim
    • M CSF
    • M-CSF
    • Macrophage colony stimulating factor
    • Macrophage Colony Stimulating Factor 1
    • MCSF
    • MGC31930
    • Processed macrophage colony-stimulating factor 1
    see all
  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications
    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localizationCell membrane and Secreted > extracellular space.
  • Information by UniProt

References for Recombinant rat M-CSF protein (Active) (ab56288)

ab56288 has not yet been referenced specifically in any publications.

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