Overview

  • Product name
    Recombinant rat RAB1B protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Rat

Specifications

Our Abpromise guarantee covers the use of ab90789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: 100 pmol of protein can bind > 80 pmol of GDP.
  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, 200mM Sodium chloride, 2mM Magnesium chloride, 2mM Beta mercaptoethanol, 0.01mM GDP, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • RAB1B
    • RAB1B member RAS oncogene family
    • RAB1B_HUMAN
    • Ras related protein Rab1B
    • Ras-related protein Rab-1B
    • Small GTP binding protein
    see all
  • Function
    Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Post-translational
    modifications
    Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
    AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.
  • Cellular localization
    Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.
  • Information by UniProt

References

ab90789 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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