Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesRat

Specifications

Our Abpromise guarantee covers the use of ab90610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: 100 pmol of protein can bind > 80 pmol of GDP.
  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 25mM HEPES, 200mM Sodium chloride, 2mM DTE (1, 4-Dithioerythritol), 10mM Magnesium chloride, 0.01mM GDP, pH 7.2

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • GS2
    • GTP-binding protein Ram
    • HsT18676
    • MGC117246
    • Mutant Ras related protein Rab-27A
    • Rab-27
    • RAB-27A
    • RAB27
    • RAB27A
    • RAB27A member RAS oncogene family
    • RAM
    • Ras-related protein Rab-27A
    • Ras-related protein Rab27A
    • RB27A_HUMAN
    see all
  • FunctionPlays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
  • Tissue specificityFound in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
  • Involvement in diseaseDefects in RAB27A are a cause of Griscelli syndrome type 2 (GS2) [MIM:607624]. Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationMembrane. Melanosome. Late endosome. Lysosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.
  • Information by UniProt

References for Recombinant rat RAB27A protein (ab90610)

ab90610 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab90610.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"