Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Rat

Specifications

Our Abpromise guarantee covers the use of ab90028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: 100 pmol of protein can bind > 80 pmol of GDP.
  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, 2mM Beta mercaptoethanol, 100mM Sodium chloride, 10mM Magnesium chloride, 0.01mM GDP, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Rab 3A
    • RAB 3A member RAS oncogene family
    • Rab3a
    • RAB3A member RAS oncogene family
    • RAB3A_HUMAN
    • RAS associated protein RAB 3A
    • RAS associated protein RAB3A
    • Ras related protein Rab 3A
    • Ras related protein Rab3A
    • Ras-related protein Rab-3A
    see all
  • Function
    Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
  • Tissue specificity
    Specifically expressed in brain.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Images

  • Anti-Rab3A antibody (ab80192) at 1/250 dilution + Recombinant rat Rab3A protein (ab90028) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Last lane on right hand side: Rab3A protein (Active) (ab90028) used in SDS-PAGE (band 27kDa).

References

ab90028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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