The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological Activity:
ab78608 is fully biologically active when compared to standard. The activity is determined by the ability to chemoattract total human lymphocytes and mouse T-cells at a concentration between 1-10ng/ml.
% SDS-PAGE. Purity is greater than 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE.
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Reconstituted material should be aliquoted and frozen at -20C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Beta chemokine RANTES
Beta chemokine RANTES precursor
C C motif chemokine 5
Chemokine (C C motif) ligand 5
Chemokine CC Motif Ligand 5
Eosinophil chemotactic cytokine
Regulated upon activation normally T expressed and presumably secreted
Small inducible cytokine A5
Small inducible cytokine A5 (RANTES)
Small inducible cytokine subfamily A (Cys Cys) member 5
Small-inducible cytokine A5
T cell specific protein p288
T cell specific RANTES protein
T cell-specific protein P228
T-cell-specific protein RANTES
FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificityT-cell and macrophage specific.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
Post-translational modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells. The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
ab78608 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Rat RANTES has a predicted MW of 7.8 kDa.
References for Recombinant rat RANTES protein (ab78608)
has not yet been referenced specifically in any publications.
Publishing research using ab78608? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"