Recombinant rat TNF alpha protein (ab9756)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP16599
    • SpeciesRat
    • SequenceMLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
    • Amino acids80 to 235
    • Additional sequence informationCorresponds to the mature soluble, biologically active form.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLyophilised
  • Additional notesThe ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.05 ng/ml, corresponding to a specific activity of > 5 x 107 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all
  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt

References for Recombinant rat TNF alpha protein (ab9756)

ab9756 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Functional Studies
Rat heart was perfused 30min with Tyrode and TNF-a at 20ng/ml, using Langendorff system.
Username

Mr. Baptiste Jude

Verified customer

Submitted Oct 07 2015

For lot 851145: Centrifuge the vial prior to opening. Reconstitute in 200ul sterile water to a concentration of 0.1 mg/ml (or add 20ul for 1mg/ml). Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is ...

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I am terribly sorry about the delay replying to you regarding ab9756, I have had trouble contacting the source of this protein. I have just received confirmation that the protein gave only 1 band when tested in WB with the antibody ab9755. It is not li...

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Thank you very much for your patience, I have just heard back from the originator of these proteins. Ab9756: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"