Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Molecular weight28 kDa
    • Additional sequence informationThe protein is a 27.6 kDa monomer containing a N-terminus 6x His-Tag.

Specifications

Our Abpromise guarantee covers the use of ab51993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Flow Cytometry

  • Purity> 95 % SDS-PAGE.
    The recombinant RFP (Red Fluorescence Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence of the protein.
    Endotoxin was removed for in vivo injection or cell culture applications. Endotoxin level equal to or less than 0.1 ng/µg.
  • FormLyophilised
  • Additional notes

    This protein (DSRed) is suitable as control reagent for RFP expression studies or as a labeling reagent.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • ReconstitutionReconstitute with 100 µl dH2O to 1mg/ml

General Info

  • Alternative names
    • DsRed
    • GFP like chromoprotein
    • red fluorescent protein
    • RFP
  • RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Recombinant RFP protein images

  • ab51993 loaded under reducing conditions and stained with coomassive blue.

References for Recombinant RFP protein (ab51993)

This product has been referenced in:
  • Dufour YS  et al. Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLoS Comput Biol 12:e1005041 (2016). Read more (PubMed: 27599206) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"