Recombinant S. cerevisiae AOS1+UBA2 protein (Chemical Free) (ab80763)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Saccharomyces cerevisiae

Specifications

Our Abpromise guarantee covers the use of ab80763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Conjugation

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes

    Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM HEPES, 150mM Sodium chloride, pH 8.0

General Info

  • Alternative names
    • SAE1
    • SAE2
    • SUMO 1 activating enzyme subunit 1
    • SUMO1 activating enzyme subunit 2
    • Ubiquitin-like 1-activating enzyme E1A
    • Ubiquitin-like 1-activating enzyme E1B
    • UBLE1A
    • UBLE1B
    see all
  • Relevance
    E1-SUMO protein is a heterodimer consisted of human AOS1 and UBA2. The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
  • Cellular localization
    Nuclear

References

ab80763 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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