Recombinant S. cerevisiae GAL4 protein (ab81879)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesSaccharomyces cerevisiae
    • Amino acids1 to 147

Specifications

Our Abpromise guarantee covers the use of ab81879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.
  • FormLiquid
  • Additional notes


    Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • Antigen NY CO 27
    • Antigen NY-CO-27
    • Antigen NYCO27
    • GAL 4
    • Gal-4
    • GAL4
    • Galectin 4
    • Galectin-4
    • Galectin4
    • Homo sapiens galectin4 mRNA complete cds
    • L 36 lactose binding protein
    • L-36 lactose-binding protein
    • L36 lactose binding protein
    • L36LBP
    • Lactose binding lectin 4
    • Lactose-binding lectin 4
    • Lectin galactoside binding soluble 4
    • LEG4_HUMAN
    • LGALS4
    see all
  • FunctionGalectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
  • Sequence similaritiesContains 2 galectin domains.
  • DomainContains two homologous but distinct carbohydrate-binding domains.
  • Information by UniProt

References for Recombinant S. cerevisiae GAL4 protein (ab81879)

ab81879 has not yet been referenced specifically in any publications.

Product Wall

The GAL4 –AH (P1018) has a fragment 1-147 aa and an additiona alpha helix sequence (denoted AH).

Please find the answer from the lab and the attached data:

"The protein contains no tag but an alpha-helix peptide on its C-terminus. Yes, the protein is active for gel shift experiment. Attached please find the gel shift experimental dat...

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In the absence of the alpha helix, the GAL4 protein willbind to DNA but will not activate transcription. The alpha helix allows the protein complex to activate genes involved in galactose metabolism.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"