Recombinant S. japonicum GST protein (ab89494)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Schistosoma japonicum
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
    • Molecular weight
      28 kDa
    • Amino acids
      1 to 244
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab89494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab89494 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Glutathione S Transferase
    • Glutathione S-transferase class-mu 26 kDa isozyme
    • GST
    • Sj26 antigen
    • SjGST
    see all
  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Images

  • 15% SDS-PAGE showing ab89494 at approximately 28.3kDa (3µg).

References

ab89494 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry.

We do not sell this component separately. However, I can suggest you could consider using ab89494 as standard protein for use with ab128573 6XHis-tag ELISA Kit. I can recommend reviewing the online datasheet for f...

Read More

I’ve attached the supporting SDS-PAGE image (3ug of protein) of ab89494, from the lab. As you can verify, it came out clear.

As mentioned yesterday, we also re-verified its concentration, ie. 1 mg/ml (determined by Bradford assay).
Read More

I have heard back from the supplier and they have not had any other issues with that particular lot, so I am hoping it was just an issue with that particular vial of protein.

A free of charge replacement should arrive tomorrow for you and you...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up