Recombinant S. japonicum GST protein (ab89494)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP08515
    • SpeciesSchistosoma japonicum
    • SequenceMGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
    • Molecular weight28 kDa
    • Amino acids1 to 244
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab89494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab89494 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Glutathione S Transferase
    • Glutathione S-transferase class-mu 26 kDa isozyme
    • GST
    • Sj26 antigen
    • SjGST
    see all
  • RelevanceGST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Recombinant S. japonicum GST protein images

  • 15% SDS-PAGE showing ab89494 at approximately 28.3kDa (3µg).

References for Recombinant S. japonicum GST protein (ab89494)

ab89494 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

We do not sell this component separately. However, I can suggest you could consider using ab89494 as standard protein for use with ab128573 6XHis-tag ELISA Kit. I can recommend reviewing the online datasheet for f...

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I’ve attached the supporting SDS-PAGE image (3ug of protein) of ab89494, from the lab. As you can verify, it came out clear.

As mentioned yesterday, we also re-verified its concentration, ie. 1 mg/ml (determined by Bradford assay).
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I have heard back from the supplier and they have not had any other issues with that particular lot, so I am hoping it was just an issue with that particular vial of protein.

A free of charge replacement should arrive tomorrow for you and you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"