Recombinant SARS CoV Envelope protein (ab63311)

Overview

  • Product nameRecombinant SARS CoV Envelope protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SequenceMRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA

Specifications

Our Abpromise guarantee covers the use of ab63311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Acetate buffer, pH 4.0

  • ReconstitutionAdd 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names
    • E
    • E protein
    • Envelope small membrane protein
    • sM protein
  • RelevanceComponent of the viral envelope that plays a central role in virus morphogenesis and assembly. May be sufficient to form virus-like particles
  • Cellular localizationVirion membrane; Single-pass type III membrane protein (Potential). Golgi apparatus membrane; Single-pass type III membrane protein (Potential). Note: Largely membrane-embedded. The N-terminus could be located within the membrane near the cytoplasmic side since no part of the protein is aparently exposed on the virion outside. The large hydrophobic domain could form a hairpin, looping back through the membrane. There also may be a second hydrophobic domain integrated into membranes.

Recombinant SARS CoV Envelope protein images

  • 1. MW marker – 14, 21, 31, 45, 66, 97 kDa
    2. reduced and heated sample, 5µg/lane
    3. non-reduced and non-heated sample, 5µg/lane

References for Recombinant SARS CoV Envelope protein (ab63311)

ab63311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"