Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesSheep
    • SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.
    • Amino acids24 to 195

Specifications

Our Abpromise guarantee covers the use of ab73378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 107 IU/mg.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab73378 was purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by both RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile 18MOhm/cm H2O to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • Antiluteolysin
    • IFN-epsilon
    • IFNE
    • IFNE_HUMAN
    • IFNE1
    • IFNT1
    • Interferon epsilon
    • Interferon epsilon 1
    • Interferon epsilon-1
    • Interferon tau 1
    • OTP
    • PRO655
    • TP1
    • Trophoblastin
    see all
  • Tissue specificityExpressed in prostate cancer cell line PC-3, amnion-derived WISH cells, melanoma cell line SK-MEL-28, cell line Daudi and very weakly in MCF-7 human breast cancer cells. Not detected in cell lines Namalwa (Burkitt's lymphoma) and Jurkat (T-cell).
  • Sequence similaritiesBelongs to the alpha/beta interferon family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant sheep IFNE1 protein (ab73378)

ab73378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"