Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein (ab82118)

Overview

  • Product nameRecombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • AccessionP03070
    • SpeciesSimian Virus 40 (SV40)
    • SequenceMDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEE KMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNE ENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAV FSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTP HRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPG GLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFE MCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRV DSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPK MDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNV NLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDY LDGSVKVNLEKKHLNKRTQIFPPGIVTMNEFSVPKTLQARFVKQIDFRAK DYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWK ERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNED GGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKK PPTPPPEPET
    • Amino acids1 to 708
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab82118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Other

  • Purity> 95 % Affinity purified.
    ab82118 is greater than 95% homogeneous based on SDS-PAGE analysis, purified by affinity and FPLC chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • Large T antigen
    • LT
    • LTAG
    • Simian virus 40
  • RelevanceSV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
  • Cellular localizationHost nucleus

References for Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein (ab82118)

ab82118 has not yet been referenced specifically in any publications.

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