Recombinant Streptavidin protein (ab123480)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Molecular weight56 kDa

Specifications

Our Abpromise guarantee covers the use of ab123480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe activity of recombinant Streptavidin is ~17 units/mg, where one unit is defined as the amount of protein required to bind 1.0 µg of D- biotin.
  • Applications

    Western blot

    Flow Cytometry

    In situ hybridization

    SDS-PAGE

  • FormLyophilised
  • Additional notesThe extinction coefficient of Streptavidin is E1% 280=32.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute the powder with desired buffer. The reconstituted solution will be stable for at least six months at -20°C The reconstituted solution may also be divided into aliquots to avoid repeated freeze-thaw cycles.

General Info

  • Alternative names
    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localizationCytoplasmic

References for Recombinant Streptavidin protein (ab123480)

ab123480 has not yet been referenced specifically in any publications.

Product Wall


ab123480 is the full length streptavidin protein and actively binds biotin.

The laboratory has not had experience using streptavidin protein, followed by primary anti-streptavidin and secondary labeling via western blot. They suggested us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"