Recombinant Streptavidin protein (ab123480)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Molecular weight
      56 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab123480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The activity of recombinant Streptavidin is ~17 units/mg, where one unit is defined as the amount of protein required to bind 1.0 µg of D- biotin.
  • Applications

    Western blot

    Flow Cytometry

    In situ hybridization

    SDS-PAGE

  • Form
    Lyophilised
  • Additional notes
    The extinction coefficient of Streptavidin is E1% 280=32.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute the powder with desired buffer. The reconstituted solution will be stable for at least six months at -20°C The reconstituted solution may also be divided into aliquots to avoid repeated freeze-thaw cycles.

General Info

  • Alternative names
    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • Relevance
    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization
    Cytoplasmic

References

ab123480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


ab123480 is the full length streptavidin protein and actively binds biotin.

The laboratory has not had experience using streptavidin protein, followed by primary anti-streptavidin and secondary labeling via western blot. They suggested us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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