Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SequenceMVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
    • Molecular weight17 kDa including tags
    • Amino acids25 to 183
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab78833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab78833 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Tris, pH 7.5

General Info

  • Alternative names
    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localizationCytoplasmic

Recombinant Streptavidin protein images

  • 15% SDS-PAGE showing ab78833 at approximately 17kDa (3µg).

References for Recombinant Streptavidin protein (ab78833)

This product has been referenced in:
  • Qin GW et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications Journal of Nanoparticle Research 13:45-51 (2010). ELISA . Read more (PubMed: ) »

See 1 Publication for this product

Product Wall

We weren’t sure what your client means by “accessible”. If they’re asking whether it’s detectable with a His tag antibody, then in principle it should be. If they are asking whether it’s the cleavable form, regrettab...

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I can confirm that the His tag is functional in this peptide for complexing with Nickel. The Met and Val in front of the His tag helps the His tag for proper configuration and has no impact on the protein’s functionality. I hope this information is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"