Recombinant thermus aquaticus RecA protein (ab123228)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, Electron Microscopy
Description
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Product name
Recombinant thermus aquaticus RecA protein
See all RecA proteins and peptides -
Biological activity
The activity of single-stranded DNA-dependent ATPase was confirmed.
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Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Thermus aquaticus -
Predicted molecular weight
37 kDa -
Amino acids
1 to 340 -
Additional sequence information
This Protein is from Thermus aquaticus
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Specifications
Our Abpromise guarantee covers the use of ab123228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Electron Microscopy
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
pH: 6.5
Constituents: 0.79% Tris HCl, 0.03% EDTA, 50% Glycerol (glycerin, glycerine), 1.17% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DNA strand exchange and recombination protein with protease and nuclease activity
- lexB
- Protein recA
see all -
Relevance
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
Cellular localization
Cytoplasmic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab123228 has been referenced in 2 publications.
- Shigemori Y et al. Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products. Nucleic Acids Res 33:e126 (2005). PubMed: 16087733
- Angov E & Camerini-Otero RD The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization. J Bacteriol 176:1405-12 (1994). PubMed: 8113181