Anti-Recoverin antibody [6A55CD6] (ab31928)

Overview

  • Product nameAnti-Recoverin antibody [6A55CD6]
    See all Recoverin primary antibodies
  • Description
    Mouse monoclonal [6A55CD6] to Recoverin
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Cow
  • Immunogen

    Recombinant full length protein of Human Recoverin.

  • Positive control
    • ab31928 gave a positive result in Mouse Retina Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IHC-FoFr Use at an assay dependent concentration. See Abreview.

Target

Anti-Recoverin antibody [6A55CD6] images

  • All lanes : Anti-Recoverin antibody [6A55CD6] (ab31928) at 2 µg/ml

    Lane 1 : Mouse Retina Whole Cell Lysate at 10 µg
    Lane 2 : Human Retina Normal Tissue Lysate at 20 µg

    Secondary
    Rabbit polyclonal to Mouse IgG H&L (HRP) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.

    See Abreview

References for Anti-Recoverin antibody [6A55CD6] (ab31928)

This product has been referenced in:
  • Redenti S  et al. Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-14 (2009). ICC/IF ; Mouse . Read more (PubMed: 19361860) »

See 1 Publication for this product

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This product is Protein G purified.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cells from cornea)
Specification cells from cornea
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 16 2010

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"