Anti-Recoverin antibody - C-terminal (ab71624)

Overview

  • Product nameAnti-Recoverin antibody - C-terminal
    See all Recoverin primary antibodies
  • Description
    Rabbit polyclonal to Recoverin - C-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C terminal region of human Recoverin conjugated to KLH

  • Positive control
    • Y79 cell lysate and human hepatocarcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71624 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).

Target

  • FunctionSeems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
  • Tissue specificityRetina and pineal gland.
  • Sequence similaritiesBelongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • 23 kDa photoreceptor cell-specific protein antibody
    • Cancer associated retinopathy protein antibody
    • Cancer-associated retinopathy protein antibody
    • CAR antibody
    • CAR protein antibody
    • p26 antibody
    • Protein CAR antibody
    • RCV1 antibody
    • RCVRN antibody
    • RECO_HUMAN antibody
    • Recoverin antibody
    • S-modulin antibody
    see all

Anti-Recoverin antibody - C-terminal images

  • Anti-Recoverin antibody - C-terminal (ab71624) at 1/100 dilution + Y79 cell lysate at 12.5 µg

    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab71624, at 1/50 dilution, staining Recoverin in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71624 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-Recoverin antibody - C-terminal (ab71624)

ab71624 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"