Anti-Recoverin antibody - C-terminal (ab71624)

Overview

  • Product name
    Anti-Recoverin antibody - C-terminal
    See all Recoverin primary antibodies
  • Description
    Rabbit polyclonal to Recoverin - C-terminal
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C terminal region of human Recoverin conjugated to KLH

  • Positive control
    • Y79 cell lysate and human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab71624 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
  • Tissue specificity
    Retina and pineal gland.
  • Sequence similarities
    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • 23 kDa photoreceptor cell-specific protein antibody
    • Cancer associated retinopathy protein antibody
    • Cancer-associated retinopathy protein antibody
    • CAR antibody
    • CAR protein antibody
    • p26 antibody
    • Protein CAR antibody
    • RCV1 antibody
    • RCVRN antibody
    • RECO_HUMAN antibody
    • Recoverin antibody
    • S-modulin antibody
    see all

Images

  • Anti-Recoverin antibody - C-terminal (ab71624) at 1/100 dilution + Y79 cell lysate at 12.5 µg

    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab71624, at 1/50 dilution, staining Recoverin in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71624 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References

ab71624 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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