Overview

  • Product name
  • Description
    Rabbit polyclonal to RECQ1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 400-649 of Human RECQ1 (Uniprot: P46063).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and NIH 3T3 whole cell lysates; HeLa cells; HumanU87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ATP dependent DNA helicase Q1 antibody
    • DNA helicase Q1 like antibody
    • DNA helicase RecQ like type 1 antibody
    • RECQ L1 antibody
    • RecQ protein like antibody
    • RecQ protein like 1 antibody
    • RECQL antibody
    • RECQL1 antibody
    see all

Images

  • Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 72 kDa



    7.5% SDS PAGE
  • Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 72 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling RECQ1 with ab151501 at 1/100 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling RECQ1 with ab151501 at 1/100 dilution.

References

ab151501 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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