Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RECQL5 with ab91422 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - RECQL5 antibody (ab91422)
All lanes : Anti-RECQL5 antibody (ab91422) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg
Predicted band size : 109 kDa
Immunoprecipitation - RECQL5 antibody (ab91422)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91422. 20% of the immunoprecipitate was loaded per lane, and probed with ab91422 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 10 seconds.
Patterson K et al. Altered RECQL5 expression in urothelial bladder carcinoma increases cellular proliferation and makes RECQL5 helicase activity a novel target for chemotherapy. Oncotarget7:76140-76150 (2016).
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