Overview

  • Product name
  • Description
    Rabbit polyclonal to RED
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide designed between residues 507 and 557 of Human RED (NP_006074.2)

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May bind to chromatin.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the RED family.
  • Developmental stage
    Expressed at similar levels in fetal and adult tissues.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • chondrosarcoma associated protein 2 antibody
    • CSA2 antibody
    • Cytokine IK antibody
    • IK antibody
    • IK cytokine down regulator of HLA II antibody
    • IK factor antibody
    • IK Gene antibody
    • prer protein antibody
    • Protein Red antibody
    • Protein RER antibody
    • RD element antibody
    • RED protein antibody
    • RED_HUMAN antibody
    • RER antibody
    see all

Images

  • All lanes : Anti-RED antibody (ab85965) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 66 kDa


    Exposure time : 10 seconds
  • 1mg HeLa whole cell lysate was immunoprecipitated using 3µg ab85965.
    20% of IP was loaded per lane and probed with with ab85965 at 1 µg/ml (lane 1) and with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 10 seconds.

References

ab85965 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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