Anti-RED1 antibody (ab89436)
Key features and details
- Mouse polyclonal to RED1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RED1 antibody
See all RED1 primary antibodies -
Description
Mouse polyclonal to RED1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human RED1.
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Positive control
- Human kidney tissue lysate, A431 cell lysate, RED1 transfected 293T cell line lysate and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis. -
Tissue specificity
Highly expressed in brain and heart and at lower levels in placenta. Fair expression in lung, liver and kidney. Detected in brain, heart, kidney, lung and liver (at protein level). Isoform 5 is high expressed in hippocampus and colon. Isoform 5 is expressed in pediatric astrocytomas and the protein has a decreased RNA-editing activity. The decrease in RNA editing correlates with the grade of malignancy of the tumors, with the high grade tumors showing lower editing is seen. -
Sequence similarities
Contains 1 A to I editase domain.
Contains 2 DRBM (double-stranded RNA-binding) domains. -
Cellular localization
Nucleus. Nucleus > nucleolus. Shuttles between nucleoli and the nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 104 Human
- Omim: 601218 Human
- SwissProt: P78563 Human
- Unigene: 474018 Human
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Alternative names
- ADARB 1 antibody
- ADARB1 antibody
- 1700057H01Rik antibody
see all
Images
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Anti-RED1 antibody (ab89436) at 1 µg/ml + Human kidney lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 81 kDa -
Anti-RED1 antibody (ab89436) at 1 µg/ml + A431 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RED1 antibody (ab89436) at 1 µg/ml
Lane 1 : RED1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands. -
ab89436 at 10 µg/ml staining RED1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab89436 has not yet been referenced specifically in any publications.