Overview

  • Product nameAnti-Reelin antibody [G10]
    See all Reelin primary antibodies
  • Description
    Mouse monoclonal [G10] to Reelin
  • Tested applicationsSuitable for: WB, IHC (PFA fixed), ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin

  • Positive control
    • Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.

Properties

Applications

Our Abpromise guarantee covers the use of ab78540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 388 kDa.
IHC (PFA fixed) 1/1000 - 1/2000.
ICC Use at an assay dependent concentration.

Target

  • FunctionExtracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
  • Tissue specificityAbundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.
  • Involvement in diseaseLissencephaly 2
  • Sequence similaritiesBelongs to the reelin family.
    Contains 16 BNR repeats.
    Contains 8 EGF-like domains.
    Contains 1 reelin domain.
  • Developmental stageExpressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.
  • DomainThe basic C-terminal region is essential for secretion.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIS2 antibody
    • PRO1598 antibody
    • Reeler antibody
    • Reelin antibody
    • RELN antibody
    • RELN_HUMAN antibody
    • RL antibody
    see all

Anti-Reelin antibody [G10] images

  • ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.
  • Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.
  • Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size : 388 kDa
    Observed band size : 388 kDa
  • All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain membrane lysate

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size : 388 kDa
    Observed band size : 388 kDa
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Reelin antibody [G10] (ab78540)

This product has been referenced in:
  • Murthy S  et al. Serotonin receptor 3A controls interneuron migration into the neocortex. Nat Commun 5:5524 (2014). IHC ; Mouse . Read more (PubMed: 25409778) »
  • McCullough SD  et al. Reelin is a target of polyglutamine expanded ataxin-7 in human spinocerebellar ataxia type 7 (SCA7) astrocytes. Proc Natl Acad Sci U S A 109:21319-24 (2012). WB, ChIP ; Human . Read more (PubMed: 23236151) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Mouse Cultured Cells (Primary cortical cells)
Specification Primary cortical cells
Permeabilization Yes - Acetone - 10 minutes
Fixative Paraformaldehyde
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Verified customer

Submitted Jul 24 2013

Merci de nous avoir contactés.

Comme convenu j'ai mis en place l'envoi d'une unité de ab18570 en remplacement du ab78540, le numéro de commande de remplacement gratuit de ce produit est ***.
Vous recevrez prochainement...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"