Anti-Reelin antibody [G10] (ab78540)
Key features and details
- Mouse monoclonal [G10] to Reelin
- Suitable for: WB, IHC (PFA fixed)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Reelin antibody [G10]
See all Reelin primary antibodies -
Description
Mouse monoclonal [G10] to Reelin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC (PFA fixed)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Mouse Reelin aa 150-500.
Database link: Q60841 -
Positive control
- Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.242% Tris -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
G10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 388 kDa.
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 388 kDa. |
IHC (PFA fixed)
Use at an assay dependent concentration. |
Target
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Function
Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. -
Tissue specificity
Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver. -
Involvement in disease
Lissencephaly 2 -
Sequence similarities
Belongs to the reelin family.
Contains 16 BNR repeats.
Contains 8 EGF-like domains.
Contains 1 reelin domain. -
Developmental stage
Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum. -
Domain
The basic C-terminal region is essential for secretion. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 5649 Human
- Entrez Gene: 19699 Mouse
- Entrez Gene: 24718 Rat
- Omim: 600514 Human
- SwissProt: P78509 Human
- SwissProt: Q60841 Mouse
- SwissProt: P58751 Rat
- Unigene: 655654 Human
see all -
Alternative names
- LIS2 antibody
- PRO1598 antibody
- Reeler antibody
see all
Images
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ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.
This image was generated using the ascites version of the product.
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All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain membrane lysate
Secondary
All lanes : Goat anti-mouse secondary antibody
conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.
This image was generated using the ascites version of the product.
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Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg
Secondary
Goat anti-mouse secondary
antibody conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDaThis image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab78540 has been referenced in 30 publications.
- Ardalan M et al. Reelin cells and sex-dependent synaptopathology in autism following postnatal immune activation. Br J Pharmacol 179:4400-4422 (2022). PubMed: 35474185
- Mercier MS et al. Long-term potentiation in neurogliaform interneurons modulates excitation-inhibition balance in the temporoammonic pathway. J Physiol 600:4001-4017 (2022). PubMed: 35876215
- Caramello A et al. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife 10:N/A (2021). PubMed: 33393905
- Gavrilovici C et al. Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders. Cereb Cortex Commun 2:tgaa096 (2021). PubMed: 33615226
- Kang ZC et al. Pinocembrin Ameliorates Cognitive Impairment Induced by Vascular Dementia: Contribution of Reelin-dab1 Signaling Pathway. Drug Des Devel Ther 14:3577-3587 (2020). PubMed: 32943845