Overview

  • Product nameAnti-REEP5 antibody
    See all REEP5 primary antibodies
  • Description
    Mouse monoclonal to REEP5
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
  • Immunogen

    Recombinant fragment: KCGFLLWCMA PSPSNGAELL YKRIIRPFFL KHESQMDSVV KDLKDKAKET ADAITKEAKK ATVNLLGEEK KS, corresponding to amino acids 113-185 of Human REEP5, with tag

Properties

Applications

Our Abpromise guarantee covers the use of ab76451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
    Note: This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMay promote functional cell surface expression of olfactory receptors.
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • C5orf18 antibody
      • D5S346 antibody
      • Deleted in polyposis 1 antibody
      • DP1 antibody
      • Polyposis coli region hypothetical protein DP1 antibody
      • Polyposis locus protein 1 antibody
      • Protein TB2 antibody
      • Receptor accessory protein 5 antibody
      • Receptor expression enhancing protein 5 antibody
      • TB2 antibody
      • TB2 protein antibody
      • YOP1 antibody
      see all

    Anti-REEP5 antibody images

    • Anti-REEP5 antibody (ab76451) at 1/500 dilution + Recombinant protein at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 21 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    References for Anti-REEP5 antibody (ab76451)

    ab76451 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Xenopus laevis Cell (meiotic egg extract)
    Specification meiotic egg extract
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
    Loading amount 25 µg
    Specification meiotic egg extract
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"