Overview

  • Product name
  • Description
    Rabbit polyclonal to REG1 alpha
  • Specificity
    This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity.
  • Tested applications
    Suitable for: IHC-P, ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human REG 1 alpha. A 17.8 kDa protein containing 144 amino acid residues of the human REG 1 alpha and 12 additional amino acid residues, HisTag. Source of immunogen E.coli

  • Positive control
    • recombinant protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).

Target

  • Function
    Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
  • Tissue specificity
    In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Developmental stage
    High expression levels in fetal and infant brains; much lower in adult brains.
  • Post-translational
    modifications
    The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ICRF antibody
    • Islet cells regeneration factor antibody
    • Islet of Langerhans regenerating protein antibody
    • Lithostathine 1 alpha antibody
    • Lithostathine 1 alpha precursor antibody
    • Lithostathine antibody
    • Lithostathine-1-alpha antibody
    • MGC12447 antibody
    • P19 antibody
    • Pancreatic stone protein antibody
    • Pancreatic stone protein secretory antibody
    • Pancreatic thread protein antibody
    • Protein X antibody
    • PSP antibody
    • PSPS antibody
    • PSPS1 antibody
    • PTP antibody
    • RATRGPI antibody
    • REG antibody
    • REG-1-alpha antibody
    • Reg1 antibody
    • REG1A antibody
    • REG1A_HUMAN antibody
    • Regenerating islet derived 1 alpha antibody
    • Regenerating islet derived 1 alpha pancreatic stone protein pancreatic thread protein antibody
    • Regenerating islet-derived protein 1-alpha antibody
    • Regenerating protein I alpha antibody
    see all

Images

  • Anti-REG1 alpha antibody (ab47099) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa


    Exposure time : 30 seconds

References

This product has been referenced in:
  • Radon TP  et al. Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma. Clin Cancer Res 21:3512-21 (2015). Read more (PubMed: 26240291) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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