Overview

  • Product name
    Anti-REG4 antibody [MM0524-9B21]
    See all REG4 primary antibodies
  • Description
    Mouse monoclonal [MM0524-9B21] to REG4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human REG4 protein.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human colon Adenocarcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by protein G affinity chromatography and 0.2 µm filtered.
  • Clonality
    Monoclonal
  • Clone number
    MM0524-9B21
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.

Target

  • Function
    May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
  • Tissue specificity
    Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly expressed in most colorectal tumors than in normal colon. Preferentially expressed in mucinous tumors and in some cases neuro-endocrine tumors. Expressed in mucus-secreting cells and enterocyte-like cells. In small intestine expressed at the basal perinuclear zone of goblet cells.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastrointestinal secretory protein antibody
    • GISP antibody
    • Reg IV antibody
    • REG like protein antibody
    • REG-4 antibody
    • REG-like protein antibody
    • Reg4 antibody
    • REG4_HUMAN antibody
    • Regenerating gene type IV antibody
    • Regenerating islet derived family member 4 antibody
    • Regenerating islet derived protein 4 precursor antibody
    • Regenerating islet-derived protein 4 antibody
    • Regenerating islet-derived protein IV antibody
    • REGIV antibody
    • RELP antibody
    see all

Images

  • IHC image of REG4 staining in Human colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89917, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab89917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab89917.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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