• Product nameAnti-Regucalcin antibody [17]
    See all Regucalcin primary antibodies
  • Description
    Mouse monoclonal [17] to Regucalcin
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human), expressed in E. coli.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Proclin
    Constituents: Tris HCl, Sodium citrate, pH 7.0
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number17
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab67336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ELISA Use at an assay dependent concentration.


  • FunctionGluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities.
  • Sequence similaritiesBelongs to the SMP-30/CGR1 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gluconolactonase antibody
    • GNL antibody
    • RC antibody
    • Regucalcin antibody
    • RGN antibody
    • RGN_HUMAN antibody
    • Senescence marker protein 30 antibody
    • SMP 30 antibody
    • SMP-30 antibody
    see all

Anti-Regucalcin antibody [17] images

  • Anti-Regucalcin antibody [17] (ab67336) at 1/1000 dilution + Human recombinant Regucalcin protein

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • IHC image of aRegucalcin staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab67336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab67336, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Regucalcin antibody [17] (ab67336)

ab67336 has not yet been referenced specifically in any publications.

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