Overview

  • Product name
    Anti-Rel B antibody [EP613Y]
    See all Rel B primary antibodies
  • Description
    Rabbit monoclonal [EP613Y] to Rel B
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Rel B aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Raji and Daudi cell lysates. IHC-P: Human lymphoma tissue. Flow Cyt: Daudi cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab33917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/100 - 1/250.
IP 1/50.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/2000.

Target

  • Function
    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
  • Sequence similarities
    Contains 1 RHD (Rel-like) domain.
  • Domain
    Both N- and C-terminal domains are required for transcriptional activation.
  • Post-translational
    modifications
    Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • I REL antibody
    • I-Rel antibody
    • IREL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • relB antibody
    • RELB_HUMAN antibody
    • Reticuloendotheliosis viral oncogene homolog B antibody
    • Transcription factor Rel B antibody
    • Transcription factor RelB antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog B antibody
    • v rel reticuloendotheliosis viral oncogene homolog B antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human lymphoma using ab33917
  • All lanes : Anti-Rel B antibody [EP613Y] (ab33917) at 1/500 dilution

    Lane 1 : 10ug Raji cell lysate
    Lane 2 : 10ug Daudi cell lysate

    Secondary
    Goat anti-rabbit HRP labeled.

    Predicted band size : 62 kDa
  • Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Rel B (red) with ab33917 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

References

This product has been referenced in:
  • Siak JJ  et al. The nuclear-factor kappaB pathway is activated in pterygium. Invest Ophthalmol Vis Sci 52:230-6 (2011). Read more (PubMed: 20811049) »
  • McMillan DH  et al. Lung-targeted overexpression of the NF-?B member RelB inhibits cigarette smoke-induced inflammation. Am J Pathol 179:125-33 (2011). Read more (PubMed: 21703398) »

See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab33917.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up