The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
1/100 - 1/250.
1/250 - 1/500.
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
Contains 1 RHD (Rel-like) domain.
Both N- and C-terminal domains are required for transcriptional activation.
Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
Reticuloendotheliosis viral oncogene homolog B antibody
Transcription factor Rel B antibody
Transcription factor RelB antibody
v rel avian reticuloendotheliosis viral oncogene homolog B antibody
v rel reticuloendotheliosis viral oncogene homolog B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rel B antibody [EP613Y] (ab33917)
Immunohistochemical analysis of paraffin-embedded human lymphoma using ab33917
Western blot - Anti-Rel B antibody [EP613Y] (ab33917)
All lanes : Anti-Rel B antibody [EP613Y] (ab33917) at 1/500 dilution
Lane 1 : 10ug Raji cell lysate Lane 2 : 10ug Daudi cell lysate
Secondary All lanes : Goat anti-rabbit HRP labeled.
Predicted band size: 62 kDa
Flow Cytometry - Anti-Rel B antibody [EP613Y] (ab33917)
Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Rel B (red) with ab33917 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).